The rabbit-derived antibody is purified with protein A-conjugated agarose followed by phosphorylated histone H2A.x (Ser139) peptide affinity chromatography. It specifically recognizes histone H2A.x with serine phosphorylation at Ser139.??
This product is produced by immunizing rabbits with a synthetic phosphoryl peptide corresponding to residues surrounding Ser139 of human histone H2A.x. Antibodies are purified by protein A-conjugated agarose followed by phosphorylated histone H2A.x (Ser139) peptide affinity chromatography.??
ELISA, WB, IP; Not tested in other applications
*WB=Western blotting; IP= Immunoprecipation
Recommended antibody dilution
WB: 1:2000 IP: 1:25~1:500
For Western blotting, incubate ?membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for ?two hours at room temperature with gentle shaking.??
Use at an assay dependent concentration. Optimal ?dilutions/concentrations should be determined by the end user.??
Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, ubiquitylation and newly discovered succinylation, crotonylation and 2-hydroxyisobutyrylation, etc. These modifications have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression. Histone H2A.x is a ubiquitously expressed variant of histone H2A and phosphorylation of histone H2A.x at Ser139 (termed γ-H2AX) is located in a unique C-terminal region and regulated by various kinases, including the ataxia telangiectasia-mutated (ATM) protein, the AT and Rad3-related protein, and DNA-dependent protein kinase, all of which were reported to be involved in the responses of mammalian cells to DNA double-strand breaks.??
Storage & Stability
Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.
**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.