The rabbit-derived antibody is purified with protein A-conjugated agarose followed by phosphorylated histone H2A (Thr120) peptide affinity chromatography. It specifically recognizes histone H2A with threonine phosphorylation at Thr120.
This product is produced by immunizing rabbits with a synthetic phosphoryl peptide corresponding to residues surrounding Thr120 of human histone H2A. Antibodies are purified by protein A-conjugated agarose followed by phosphorylated histone H2A (Thr120) peptide affinity chromatography.
ELISA,WB, IP; Not tested in other applications???
Recommended antibody dilution
WB: 1:2000 IP: 1:25~1:100
For Western blotting, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 with gentle shaking two hours at room temperature.??
Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user.??
Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, ubiquitylation and newly discovered succinylation, crotonylation and 2-hydroxyisobutyrylation, etc. These modifications have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression. Histone H2A phosphorylation is specifically phosphorylated during both mitosis and meiosis that is believed to be mainly involved in transcriptional activation requiring chromatin fiber decondensation, and chromosome compaction during cell division. Histone H2A phosphorylation may initiate at different phases of the cell division in different organisms, but metaphase chromosomes are always found to be heavily phosphorylated.
Storage & Stability
Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.
**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.