修飾抗體 Products
組蛋白磷酸化(phosphorylation)修飾抗體
杭州景杰生物科技有限公司
Anti-phospho-Histone H2A (Thr120) rabbit pAb

Cat#:

PTM-718

Ab Type:

P

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

2880.00

Price: (20 μl)

700.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by phosphorylated histone H2A (Thr120) peptide affinity chromatography. It specifically recognizes histone H2A with threonine phosphorylation at Thr120.


Source/Purification

This product is produced by immunizing rabbits with a synthetic phosphoryl peptide corresponding to residues surrounding Thr120 of human histone H2A. Antibodies are purified by protein A-conjugated agarose followed by phosphorylated histone H2A (Thr120) peptide affinity chromatography.


Specificity

H2A

Recommended Applications

ELISA,WB, IP; Not tested in other applications???

*WB=Western blotting?IP=Immunoprecipitation



Recommended antibody dilution

WB: 1:2000 IP: 1:25~1:100

Important

For Western blotting, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 with gentle shaking two hours at room temperature.??

Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user.??

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Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, ubiquitylation and newly discovered succinylation, crotonylation and 2-hydroxyisobutyrylation, etc. These modifications have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression. Histone H2A phosphorylation is specifically phosphorylated during both mitosis and meiosis that is believed to be mainly involved in transcriptional activation requiring chromatin fiber decondensation, and chromosome compaction during cell division. Histone H2A phosphorylation may initiate at different phases of the cell division in different organisms, but metaphase chromosomes are always found to be heavily phosphorylated.


Storage & Stability
Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.


ELISA,WB, IP; Not tested in other applications???

*WB=Western blotting?IP=Immunoprecipitation


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